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KMID : 0359319900300040459
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Korean Journal of Veterinary Research
1990 Volume.30 No. 4 p.459 ~ p.464
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Comparison of Ag - NORs stain and [ 3H ] thymidine incorporation in antiproliferative effect of caffeine on NIH3T3 cells
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Abstract
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Inhibitory effect of caffeine on NIH3T3 cell proliferation was studied by using [³H]thymidine incorporation and a modified one-step silver staining technique. The latter technique shows argyrophilic nucleolar organizer region-acsociated proteins (Ag-NORs), which are seen in nuclei as black dots. The result was compared with the counts of [³H] thymidine incorporation.
The results were summarized as follows;
1. The Ag-NORs numbers of NIH3T3 cells were 6.81±1.38 at 24 hrs, 7.13±1.26 at 48 hrs, 8.50±2.04 at l2 hrs after incubation.
2. The numbers of Ag-NORs were significantly decrease in caffeine treated groups (p<0.01).
3. The counts of [³H] thymidine incorporation were similar to the result of using Ag-NORs staining technique.
It is concluded that Ag-NOR is a rapid, simple and compatible method to quantitate cell proliferation as compared with [³H]thymidine incorporation.
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